Our new Clear+ tissue clearing system is the one strategy that delipidates samples without any change in morphology and with negligible effect on structural integrity.
Megatome can be a vibrating microtome made to section a wide array of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With substantial blade vibrating frequency and minimized blade deflection, Megatome allows large-throughput tissue sectioning with uniform surface profile, together with nominal tissue harm and knowledge reduction.
Antibodies may possibly take months to diffuse as a result of just a few millimeters of tissue, having a steep labeling gradient from floor to core.
Megatome is created for precision: the blade vibrates at a greater frequency and bigger amplitude variety than other microtomes, and contains a distinctive deflection Management system.
SE makes use of a rotational electrical industry to disperse extremely electromobile molecules (such as antibodies or surfactant micelles) in the course of a porous sample without the need of damaging electrically charged constructions throughout the tissue. This allows 2-4 day clearing of intact organs,
Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, rendering them totally transparent. This permits mild penetration in the sample and makes certain the acquisition of superior-resolution, in-aim image data.
Megatome is usually a novel microtome which allows for top-precision sectioning of a wide array of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with minimal tissue damage and knowledge reduction.
It’s the perfect time to enhance your microtome to Megatome. With precise large-frequency slicing for an unmatched selection of sample dimensions and kinds – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for numerous purposes.
Fully delipidate total mouse brains or comparably sized samples in just one working day with SmartBatch+, or in one 7 days with our passive clearing kit.
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Either obtain a launch or make a distribution zip as outlined over. Unzip the archive into a ideal megatomi.com location.
Leverage the Apparent+ tissue clearing method, together with eFLASH and patented stochastic electrotransport technologies, to swiftly obvious and label total organs. Critical highlights and options include:
Our novel SHIELD tissue preservation method types intramolecular bonds utilizing polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.